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Introduction to RNA-seq Analysis

  • Provided by: ISU, SCINet Office

In this workshop we will provide an overview of RNA-sequencing (RNA-seq) technology and best practices for experimental design. Participants will also explore preprocessing steps, read alignment using a reference genome, and differential expression analysis using DESeq2. We will also introduce tools for functional analysis and determining the biological relevance of the results.

Additionally, participants will explore the RNA-seq analysis pipeline in the absence of a reference genome using de novo transcriptome assembly.

We will also introduce pseudoalignment techniques for rapid quantification of transcript abundance without base-by-base alignment.


    • May 4, 6-7, 2026, 1 – 5 PM ET
      • Registration: Register Here
      • Prerequisites:
        • Familiarity with basic command-line concepts, next-generation sequencing data types, and have a general understanding of gene expression.