Skip to main content

RNAseq and Variant calling pipelines in Galaxy

  • Provided by: ISU, SCINet Office

In this workshop, participants will work through a complete RNA-seq analysis and a variant calling workflow using SCINet’s Galaxy interface. Participants will learn how to create workflows in Galaxy to:

  • Upload and process RNA-seq data.
  • Perform quality control, alignment, and expression quantification.
  • Identify differentially expressed genes using DESeq2.
  • Build and run a variant calling pipeline for identifying genetic variants from sequencing data

We will also explore ways in which you can share your history and workflows with other SCINet users in Galaxy and how it can be a powerful tool for collaborations.


    • June 22, 24-25, 2026, 1 – 5 PM ET
      • Registration: Register Here
      • Prerequisites:
        • Familiarity with basic command-line concepts, next-generation sequencing data types, and have a general understanding of gene expression